human cervix epithelial adeno carcinoma hela cells  (ATCC)

Journal: Plants

Article Title: Phytochemical Composition and Antioxidant Activity of a Viscum album Mother Tincture

doi: 10.3390/plants14172762

Figure Lengend Snippet: Effect on cell viability of MT-#39998 ( A , B ), and of vd-MT (from MT#39998) ( C , D ). HaCaT, BALB/c-3T3, SVT2 and HeLa cells were incubated with an increasing concentration of both extracts (0.05–5% v / v ) for 48 h. Cell viability was assessed using the MTT assay. Values are expressed as means ± SD (n ≥ 3).

Article Snippet: Immortalized murine fibroblasts BALB/c-3T3, murine fibroblasts transformed with virus SV40 (SVT2) and human cervix carcinoma cells (HeLa) were obtained from ATCC, whereas immortalized human keratinocytes (HaCaT) were obtained from Innoprot (Biscay, Spain).

Techniques: Incubation, Concentration Assay, MTT Assay

Journal: Chemical Research in Toxicology

Article Title: Aetokthonotoxin, the Causative Agent of Vacuolar Myelinopathy, Uncouples Oxidative Phosphorylation due to Protonophore Activity

doi: 10.1021/acs.chemrestox.5c00147

Figure Lengend Snippet: Impact of AETX on the mitochondrial network and membrane potential. (A) Confocal microscopy images of immunofluorescence-stained translocase of the outer mitochondrial membrane 20 protein (TOM20) (red) and DNA (blue) in HCT116 cells. Cells incubated for 24 h with (from top to bottom) 0.1% DMSO (control) or 0.1, 1, or 5 μM AETX. Scale bar 20 μm. (B–D) Violin plots with the mean indicated as a black dot and the median as a line. Standard deviation (1×) indicated as whiskers. Increasing shades of color indicate increasing AETX concentrations. (B) Intensity of the TOM20 signal as relative fluorescence units (R.F.U.) in HCT116 cells. Statistically significant difference to the control indicated with *** p < 0.001, obtained with Student's t test. Mitochondrial membrane potential measurement at different time points as the ratio of JC10 aggregate to JC10 monomer in (C) HeLa cells or (D) fibroblasts normalized to control. Statistically significant difference to the control indicated with ** p < 0.01, *** p < 0.001, obtained with Mann–Whitney (HeLa cells, 24 h incubation time) or Student's t test.

Article Snippet: Human colorectal cancer HCT116 cells (ACC 581, DMZ-German collection of Microorganisms and Cell Cultures, Germany), human cervix carcinoma HeLa cells (provided by Prof. Junker, Martin Luther University, Halle-Wittenberg, Germany), human foreskin fibroblasts CCD1092Sk cells (immortalized cell line, provided by Prof. Gekle, Julius-Berstein-Institut, Halle, Germany), and human prostate cancer PC-3 cells (purchased from ATCC, USA) were maintained at 37 °C in a humidified atmosphere with 5% CO 2 .

Techniques: Membrane, Confocal Microscopy, Immunofluorescence, Staining, Incubation, Control, Standard Deviation, Fluorescence, MANN-WHITNEY

Journal: Chemical Research in Toxicology

Article Title: Aetokthonotoxin, the Causative Agent of Vacuolar Myelinopathy, Uncouples Oxidative Phosphorylation due to Protonophore Activity

doi: 10.1021/acs.chemrestox.5c00147

Figure Lengend Snippet: Influence of AETX on oxygen consumption and ATP production rates. (A–F) Violet: HeLa cells; green: fibroblasts. Increasing shades of color indicate increasing concentrations of AETX. Standard deviation (1×) indicated as whiskers. (A–E) Square: control (0.1% DMSO), circle: 0.1 μM AETX, triangle: 1 μM AETX, inverted triangle: 2 μM AETX, diamond: 5 μM AETX. (A, B) Left-pointing triangle: 10 μM, Right-pointing triangle: 30 μM AETX. (C, D) Yellow hexagon: FCCP. Oxygen consumption rate measurement of (A) HeLa cells or (B) fibroblasts after acute stimulation with AETX. Addition of 1: AETX, 2: oligomycin, 3: FCCP, 4: rotenone/antimycin A + Hoechst 33342. Oxygen consumption rate measurement of (C) HeLa cells or (D) fibroblasts after acute stimulation with AETX or FCCP subsequent to FoF1-ATPase inhibition. Addition of 1: oligomycin, 2: AETX or 2 μM FCCP, 3: rotenone/antimycin A + Hoechst 33342. (E) Oxygen consumption rate measurement of HeLa cells and fibroblasts after 24 h of treatment with AETX. Addition of 1: oligomycin, 2: FCCP, 3: rotenone/antimycin A + Hoechst 33342. (F) ATP production rate in HeLa cells and fibroblasts respective to the origin of ATP. Statistically significant difference to the control indicated with * p < 0.05 and *** p < 0.001, obtained with Mann–Whitney (HeLa cells) or Student's t test (fibroblasts). Mean indicated as black dot and the median as line.

Article Snippet: Human colorectal cancer HCT116 cells (ACC 581, DMZ-German collection of Microorganisms and Cell Cultures, Germany), human cervix carcinoma HeLa cells (provided by Prof. Junker, Martin Luther University, Halle-Wittenberg, Germany), human foreskin fibroblasts CCD1092Sk cells (immortalized cell line, provided by Prof. Gekle, Julius-Berstein-Institut, Halle, Germany), and human prostate cancer PC-3 cells (purchased from ATCC, USA) were maintained at 37 °C in a humidified atmosphere with 5% CO 2 .

Techniques: Standard Deviation, Control, Inhibition, MANN-WHITNEY

Journal: Chemical Research in Toxicology

Article Title: Aetokthonotoxin, the Causative Agent of Vacuolar Myelinopathy, Uncouples Oxidative Phosphorylation due to Protonophore Activity

doi: 10.1021/acs.chemrestox.5c00147

Figure Lengend Snippet: Comparison of the effect of AETX, N -methyl-AETX (m-AETX, m-A), and desnitrile-AETX (dn-AETX, dn-A) on overall cell viability and mitochondrial membrane potential. (A, B) Light green: AETX, green: dn-AETX, blue: m-AETX. Standard deviation (1×) indicated as whiskers. (A) Cytotoxicity assay based on protein content (sulforhodamine B absorbance) in HCT116 cells normalized to the control. Statistically significant difference to the control indicated with ** p < 0.01 and *** p < 0.001, obtained with the Mann–Whitney test. (B) Mitochondrial membrane potential as the ratio of JC10 aggregate to JC10 monomer in treated HeLa cells normalized to the control. Mean indicated as black dot and the median as line. Statistically significant difference to treatment with AETX indicated with *** p < 0.001, obtained with Student's t test.

Article Snippet: Human colorectal cancer HCT116 cells (ACC 581, DMZ-German collection of Microorganisms and Cell Cultures, Germany), human cervix carcinoma HeLa cells (provided by Prof. Junker, Martin Luther University, Halle-Wittenberg, Germany), human foreskin fibroblasts CCD1092Sk cells (immortalized cell line, provided by Prof. Gekle, Julius-Berstein-Institut, Halle, Germany), and human prostate cancer PC-3 cells (purchased from ATCC, USA) were maintained at 37 °C in a humidified atmosphere with 5% CO 2 .

Techniques: Comparison, Membrane, Standard Deviation, Cytotoxicity Assay, Control, MANN-WHITNEY